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csa h460 rfluc lung carcinoma cell line  (ATCC)


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    ATCC csa h460 rfluc lung carcinoma cell line
    Csa H460 Rfluc Lung Carcinoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+carcinoma+cell+lines+h460/NCI-H460%3B+Lung+Carcinoma%3B+Human/pm41260900-128-17-28
    Average 95 stars, based on 304 article reviews
    csa h460 rfluc lung carcinoma cell line - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Expressing:

    Article Title: The reversion of DNA methylation-induced miRNA silence via biomimetic nanoparticles-mediated gene delivery for efficient lung adenocarcinoma therapy.
    Article Snippet: Human lung carcinoma cell lines H460, NCI-H1299, A549, and the normal human lung epithelial cell line BEAS-2B were obtained from ATCC (American Type Culture Collection, USA). hUV-MSCs and HUVECs were purchased from ScienCell Research Laboratories.. The cells were cultured in DMEM supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin in an incubator containing 5% CO2 at 37 °C.The cells were cultured in DMEM supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin in an incubator containing 5% CO2 at 37 °C.

    Article Title: The reversion of DNA methylation-induced miRNA silence via biomimetic nanoparticles-mediated gene delivery for efficient lung adenocarcinoma therapy
    Article Snippet: Human lung carcinoma cell lines H460, NCI-H1299, A549, and the normal human lung epithelial cell line BEAS-2B were obtained from ATCC (American Type Culture Collection, USA). hUV-MSCs and HUVECs were purchased from ScienCell Research Laboratories.. The cells were cultured in DMEM supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin in an incubator containing 5% CO 2 at 37 °C.The cells were cultured in DMEM supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin in an incubator containing 5% CO 2 at 37 °C.

    Quantitative RT-PCR:

    Article Title: The reversion of DNA methylation-induced miRNA silence via biomimetic nanoparticles-mediated gene delivery for efficient lung adenocarcinoma therapy.
    Article Snippet: Human lung carcinoma cell lines H460, NCI-H1299, A549, and the normal human lung epithelial cell line BEAS-2B were obtained from ATCC (American Type Culture Collection, USA). hUV-MSCs and HUVECs were purchased from ScienCell Research Laboratories.. The cells were cultured in DMEM supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin in an incubator containing 5% CO2 at 37 °C.The cells were cultured in DMEM supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin in an incubator containing 5% CO2 at 37 °C.

    Article Title: The reversion of DNA methylation-induced miRNA silence via biomimetic nanoparticles-mediated gene delivery for efficient lung adenocarcinoma therapy
    Article Snippet: Human lung carcinoma cell lines H460, NCI-H1299, A549, and the normal human lung epithelial cell line BEAS-2B were obtained from ATCC (American Type Culture Collection, USA). hUV-MSCs and HUVECs were purchased from ScienCell Research Laboratories.. The cells were cultured in DMEM supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin in an incubator containing 5% CO 2 at 37 °C.The cells were cultured in DMEM supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin in an incubator containing 5% CO 2 at 37 °C.

    Methylation:

    Article Title: The reversion of DNA methylation-induced miRNA silence via biomimetic nanoparticles-mediated gene delivery for efficient lung adenocarcinoma therapy.
    Article Snippet: Human lung carcinoma cell lines H460, NCI-H1299, A549, and the normal human lung epithelial cell line BEAS-2B were obtained from ATCC (American Type Culture Collection, USA). hUV-MSCs and HUVECs were purchased from ScienCell Research Laboratories.. The cells were cultured in DMEM supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin in an incubator containing 5% CO2 at 37 °C.The cells were cultured in DMEM supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin in an incubator containing 5% CO2 at 37 °C.

    Article Title: The reversion of DNA methylation-induced miRNA silence via biomimetic nanoparticles-mediated gene delivery for efficient lung adenocarcinoma therapy
    Article Snippet: Human lung carcinoma cell lines H460, NCI-H1299, A549, and the normal human lung epithelial cell line BEAS-2B were obtained from ATCC (American Type Culture Collection, USA). hUV-MSCs and HUVECs were purchased from ScienCell Research Laboratories.. The cells were cultured in DMEM supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin in an incubator containing 5% CO 2 at 37 °C.The cells were cultured in DMEM supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin in an incubator containing 5% CO 2 at 37 °C.



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    Image Search Results


    Illustration of the cytotoxicity activity of Ethyl 2-[5-(4-chlorophenyl)-2-methyl-1-H-Imidazole-4-yl] acetate in (A) A549 and (B) NCI-H460 cell lines.

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: Illustration of the cytotoxicity activity of Ethyl 2-[5-(4-chlorophenyl)-2-methyl-1-H-Imidazole-4-yl] acetate in (A) A549 and (B) NCI-H460 cell lines.

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Activity Assay

    SIRT6 expression in A549 and NCI-H460 NSCLC cell lines: (A) Represents the qRT-PCR analysis and (B) Represents the Western blot analysis. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. ** p < 0.01, *** p < 0.001 and **** p < 0.0001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: SIRT6 expression in A549 and NCI-H460 NSCLC cell lines: (A) Represents the qRT-PCR analysis and (B) Represents the Western blot analysis. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. ** p < 0.01, *** p < 0.001 and **** p < 0.0001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Gene Expression, Control

    The effect of imidazole derivative Ethyl 2-[5-(4-chlorophenyl)-2-methyl-1-H-Imidazole-4-yl] acetate on antioxidant regulators (A) GSH Activity, (B) GPx Activity, (C) Catalase Activity, and (D) % Radical Scavenging Activity in A549 and NCI-H460 cell lines. (Data represent mean values ±SD. * p < 0.05, ** p < 0.01, and *** p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: The effect of imidazole derivative Ethyl 2-[5-(4-chlorophenyl)-2-methyl-1-H-Imidazole-4-yl] acetate on antioxidant regulators (A) GSH Activity, (B) GPx Activity, (C) Catalase Activity, and (D) % Radical Scavenging Activity in A549 and NCI-H460 cell lines. (Data represent mean values ±SD. * p < 0.05, ** p < 0.01, and *** p < 0.001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Activity Assay

    The protein expression of cytochrome-c (cyt-c) in NSCLC cell lines A549 and NCI-H460. β-actin is used as an internal control for normalization. (Data represent mean values ±SD. ** p < 0.01, and *** p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: The protein expression of cytochrome-c (cyt-c) in NSCLC cell lines A549 and NCI-H460. β-actin is used as an internal control for normalization. (Data represent mean values ±SD. ** p < 0.01, and *** p < 0.001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Expressing, Control

    Gene and protein expression of caspase 9: (A) Caspase 9 gene expression, (B) pro-caspase 9, and (C) active-caspase 9 protein expression in A549 and NCI-H460 cell lines. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization. (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: Gene and protein expression of caspase 9: (A) Caspase 9 gene expression, (B) pro-caspase 9, and (C) active-caspase 9 protein expression in A549 and NCI-H460 cell lines. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization. (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Expressing, Gene Expression, Control

    The expression of Caspase 3: (A) Caspase 3 gene expression, (B) protein expression of pro-caspase 3, and (C) active-caspase 3 in A549 and NCI-H460 cell lines. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD.** p < 0.01, *** p < 0.001 and **** p < 0.0001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: The expression of Caspase 3: (A) Caspase 3 gene expression, (B) protein expression of pro-caspase 3, and (C) active-caspase 3 in A549 and NCI-H460 cell lines. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD.** p < 0.01, *** p < 0.001 and **** p < 0.0001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Expressing, Gene Expression, Control

    Morphological assessment of NSCLC cell lines A549 and NCI-H460 cells stained by (A) AO/EtBr, (B) Propidium iodide, (C) DCFH-DA, (D) Rhodamine-123, and (E) Hoechst 33258 were captured using a fluorescent microscope with ×20 magnification (Scale Bar - 125 μm). (F) AO/EtBr, (G) Propidium iodide, (H) DCFH-DA, (I) Rhodamine-123, and (J) Hoechst 33258 represent the analysis of morphological staining by image (J) (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: Morphological assessment of NSCLC cell lines A549 and NCI-H460 cells stained by (A) AO/EtBr, (B) Propidium iodide, (C) DCFH-DA, (D) Rhodamine-123, and (E) Hoechst 33258 were captured using a fluorescent microscope with ×20 magnification (Scale Bar - 125 μm). (F) AO/EtBr, (G) Propidium iodide, (H) DCFH-DA, (I) Rhodamine-123, and (J) Hoechst 33258 represent the analysis of morphological staining by image (J) (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Staining, Microscopy

    Gene and protein expression of Keap1 in A549 and NCI-H460 NSCLC cell lines: (A) Keap1 gene expression and (B) Western blot analysis of Keap1. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. ** p < 0.01 and *** p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: Gene and protein expression of Keap1 in A549 and NCI-H460 NSCLC cell lines: (A) Keap1 gene expression and (B) Western blot analysis of Keap1. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. ** p < 0.01 and *** p < 0.001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Expressing, Gene Expression, Western Blot, Control

    Gene and protein expression of Nrf2 in A549 and NCI-H460 NSCLC cell lines: (A) Nrf2 gene expression and (B) Western blot analysis of Nrf2. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: Gene and protein expression of Nrf2 in A549 and NCI-H460 NSCLC cell lines: (A) Nrf2 gene expression and (B) Western blot analysis of Nrf2. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Expressing, Gene Expression, Western Blot, Control